Types of Specimen Collection Containers

Blood Collection Containers – Common Vacutainer Tubes

ADDITIVE

CAP COLOUR

BLOOD VOLUME

SUITABLE FOR

Clot Activator, Plain

RED 6mL Most chemistry, including drug levels and serological tests that require serum. Also for immunohaematological tests that require clotted blood such as abnormal blood group antibody tests and cross-matching requests.

K2EDTA

PINK(GXM) 6mL For cross-matching requests only. After the tube has been filled with blood drawn by vacuum, it should be gently inverted at least 6 times to prevent clotting. Please call laboratory for update.
K2EDTA (Di-Potassium Ethylene Diamine-Tetracetate) PURPLE 3mL Most haematological tests, HbA1C, Cyclosporine, Homocysteine, and molecular genetics tests (when using blood DNA). After the tube has been filled with blood drawn by vacuum, it should be gently inverted at least 6 to 10 times to prevent clotting.
Lithium Heparin GREEN 4mL Tests requiring plasma or whole blood (such as cytogenetics when using blood DNA) and STAT biochemistry tests such as electrolytes, renal screen, ammonia test. After the tube has been filled with blood drawn by vacuum, it should be inverted gently at least 6 times to prevent clotting.
Sodium Citrate BLUE 2.7mL Coagulation studies. The ratio of blood to anticoagulant is critical for valid prothrombin time and activated partial thromboplastin time results.Allow 2.7mL of blood to be drawn by vacuum. This tube should be inverted gently at least 3-4 times in order to prevent clotting. (Must fill up to the mark)
Sodium Fluoride / Potassium Oxalate GREY 6mL Glucose tests. After the tube has been filled with blood drawn by vacuum, it should be inverted gently at least 6 times to prevent clotting.
SST II, Clot activator & serum gel separator, Plain YELLOW 5mL All tests requiring serum except those few that need red cells as well (such as abnormal blood group antibody screen, cold agglutinins). After centrifugation, the gel forms an effective barrier between the blood clot and the serum.

Other Specimen Collection Containers

TYPE OF CONTAINERS/DESCRIPTION

COMMENTS

BACTEC MYCO/F LYTIC, Culture Vial 40mL size

White-cap (aerobic)
Tube content: Supplemented Middlebrook
7H9 & Brain Heart Infusion Broth
Blood volume: 1-5mL

For fungus culture of blood and sterile body fluid only. After the bottle has been filled with blood, it should be inverted gently at least 6 times to facilitate mixing and prevent coagulation. Supplied only upon specific request.

BACTEC PEDS PLUS Blood Culture, 40mL size (ARD)

Pink-cap (aerobic)
Tube content: SPS
Blood volume: 1-3mL

For paediatric blood culture only. After the bottle has been filled with blood, it should be inverted gently at least 6 times to facilitate mixing and prevent coagulation. Supplied only upon specific request.

BACTEC PLUS Blood Culture Set, 25mL size (ARD)

Blue/Grey-cap BACTEC PLUS (aerobic) & Orange-cap BACTEC PLUS (anaerobic)
Tube content: Sodium Polyanethol
Sulfonate (SPS)
Blood volume: 3-10mL

Specific for blood culture only. After the bottle has been filled with blood, it should be inverted gently at least 6 times to facilitate mixing and prevent coagulation. Both Grey-cap & Orange-cap bottles are to be collected and sent in pairs. Supplied only upon specific request.

CULTURE SWAB with Transport Media

Sterile cotton swab attached to plastic applicator stick with transport media

For bacteriological culture from all sites. It contains a special transport media which will maintain micro-organism viability but does not promote growth of micro-organisms. Blue-cap swab is suitable for both aerobic & anaerobic organisms.

DRY SWAB without Transport Media

Sterile cotton swab attached to plastic applicator stick

For Chlamydia Trachomatis PCR test.

Sodium Heparin Tube (L.E. TUBE)

Green-cap vacutainer tube
Tube content: Sodium heparin
Blood volume: 8-10mL

For the demonstration of L.E. cells test. After the tube has been filled with blood, it should be inverted gently at least 6 times to prevent clotting.

STOOL CONTAINER

Screw cap (brown) plastic container with attached spatula

For collecting stool specimen for routine examination and culture.

URINE CONTAINER, Sterile (UNIVERSAL CONTAINER)

Sterile plastic container with screw cap

For routine urinalysis and urine culture. Also suitable for collecting body fluids and sputum for routine examination or culture.

For Genetics Department

TYPE OF SUPPLIES

COMMENTS

BOTTLE OF TRANSPORT MEDIUM

For Chorionic Villus or Products of

Conception QF-PCR

BOTTLE OF TRANSPORT MEDIUM

For Chorionic Villus or Products of

Conception Karyotyping

BOTTLE OF TRANSPORT MEDIUM

 For Bone Marrow Karyotyping

MOTTLED BLACK/TAN
Cell-Free DNA
BCT has chemical additives 10mL x 2

Non-invasive fetal aneuploidy screening (NIPS) tests using maternal blood. Avoid possible backflow from the tube. Tube contents should not touch stopper or the end of the needle during the collection procedure. Order of Draw: Perform Cell-Free DNA BCT tubes first. After the tube is filled completely, gently invert 8 to 10 times.

PLAIN STERILE UNIVERSAL BOTTLE

For Amniotic Fluid Karyotyping or QF-PCR

SALIVA KIT WITH PRESERVATIVES

For Personalised Genomics (Pathway Fit)

Miscellaneous Supplies

TYPE OF SUPPLIES/DESCRIPTION

COMMENTS

CAPILLARY TUBE  (Heparinised)

Self sealing

For neonatal serum bilirubin test. Collect 2 or 3 capillary tubes (heparinised) of blood.

CONTAINER, with Formalin

Clean container (50mL) half-filled with formalin

Supplied for transporting small histopathology specimens.

GLASS SLIDE

Rectangular 3”x1” glass slide with frosted end

Supplied for collecting pap smear and cytology smears only. Please write patient’s name on the frosted end of slide in pencil.

SLIDE HOLDER (Pap Smear Container)

Blue or white plastic holder for 2 slide wells.

Supplied with 95% alcohol for fixation of smears.

Supplied for holding cytology and pap smear glass slides only.

SPATULA (Scraper)

Wooden stick with paddle

Use to collect material for cytology smears.

SYRINGES and NEEDLES

Disposable syringes & needles

For collecting blood into non-vacutainer tubes, i.e. conventional screw-top containers.

Sizes available for syringes: 20mL, 10mL, 5mL & 2.5mL Sizes available for needles: 23G 1”, 22G 1½”, 21G 1½”

VACUTAINER NEEDLE

Green 21G vacutainer needle, regular size

Disposable vacutainer needle to be used together with vacutainer needle holder for drawing blood specimen into vacutainer tubes.

VACUTAINER NEEDLE HOLDER

White hollow plastic tube

Used to hold vacutainer needle and the vacutainer tube while collecting blood specimen.

 OTHER SUPPLIES

include non-vacutainer collection tubes, thin prep vial with cytobrush etc.

Please call 6278 9188 to request for other supplies.